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The relationships between final exam scores and the metrics of note taking activity were analyzed, and the contribution of these metrics during the course with instruction was confirmed.
Adherence to this instruction was confirmed by actigraphy.
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Briefly, the hybridization probe labeled with DIG-dUTP was synthesized using PCR DIG synthesis kit (Roche, Germany) according to the manufacturer's instructions, and labeling was confirmed by size disparity with unlabeled amplicon as a result of slower migration in agarose gel due to digoxigenin labeling.
Total RNA was extracted using RNAqueous kit (Ambion) according to the manufacturer's instructions, quality of RNA was confirmed by spectrophotometry and gel electrophoresis.
RNA was treated with DNase I (Invitrogen, instructions CA), and integrity was confirmed with a Bioanalyzer (Agilent).
The PCR product was gel purified (Qiagen, Valencia, CA) and cloned into pcDNA 2.1 vector (Invitrogen) according to the manufacturer's instructions, and the sequence was confirmed.
First, IL-6 was removed from SSc serum using a direct immunodepletion kit in accordance with the manufacturer's instructions and 60% immunodepletion was confirmed by western blotting (data not shown).
The PCR product was cloned in pENTRTM/D-TOPO® according to the manufacturer's instruction (Invitrogen) and the insert was confirmed by Sanger sequencing.
PCR products were cloned by using vector pCR 2.1-TOPO (Invitrogen, Burling, Ontario, Canada) according to the manufacturer's instructions, and the insertion size was confirmed by a second PCR.
Transposon insertions within the mutS gene were confirmed by PCR analysis following the provider's instructions, and the resulting hypermutable phenotype was confirmed by the rifampin resistance test (see below).
Mutagenesis was carried out according to the manufacturer's instructions and the presence of the mutations was confirmed by sequencing.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com