They said Ms. Briggs had failed to comply with an instruction to obtain protection orders against Mr. Bennett and also against her troubled older daughter, then 19, with whom she had repeated arguments.
Walsingham was sent to France in mid-1581 to discuss an Anglo-French alliance, but the French wanted the marriage agreed first and Walsingham was under instruction to obtain a treaty before committing to the marriage.
Chromosomal DNA obtained after proteinase treatment was amplified using GenomiPhi DNA Amplification Kit (GE Healthcare, UK) according to the manufacturer's instruction to obtain 5 μg chromosome-specific DNA.
The results from this technical examination were in addition compared with recipes and paint instructions, to obtain a better understanding of the relation between the general practice and actual painting technique of Jan Davidsz.
Symantec later published a white paper detailing PatchGuard with instructions to obtain a PatchGuard exploit.
5 µg of the purified RNA was then subjected to reverse transcription using Superscript III (Invitrogen) per manufacturer's instructions to obtain cDNAs for PCR.
The Dual-Glo Luciferase Assay System (Promega) was utilized according to the manufacturer's instructions to obtain both fLuc and Rluc8 bioluminescence measurements from an Infinite 200 plate reader (Tecan, Mannedorf, Switzerland).
EL4 cells (H-2b) [14] were transfected with the pcDNA3/HER-2 plasmid (harbouring full-length human Her2/neu from pVAX/E2A [15] and the neomycin resistance gene) using Lipofectamine 2000 reagent (Invitrogen, Carlsbad, CA, USA), according to the manufacturer's instructions, to obtain a Her2/neu positive H-2b cell line.
The immunoreaction was visualized using polymer-based MACH4 DAB Detection Kit Biocare Medicall) according to the manufacturer's instructions to obtain optimal immunoreactivity and least background artifact.
The mitochondria were lysed with RIPA Lysis Buffer (Beyotime, Shanghai, China) according to the manufacturer's instructions to obtain the cytoplasmic mitochondrial proteins.
Briefly, total RNA was amplified and labeled using the GeneChip 3′IVT express kit (Affymetrix, CA, US), according to the manufacturer's instructions, to obtain biotin labeled cRNA.
