DyLight™ 488 rat monoclonal anti-mouse CD31 (PECAM-1) antibody (Novus Biologicals Canada ULC, Oakville ON L6M 2V5, Canada) was used in immunohistochemistry at a 1 300 dilution according to the manufacturer's instructions to detect mouse CD31+ (PECAM-1) endothelial cells in the paraffin-embedded xenogafts of A2780 ovarian tumors.
MTT assay (Sigma) was performed following the manufacturer's instructions to detect cell proliferation.
The ESC supernatant was assayed by ELISA according to the manufacturer's instructions, to detect the levels of secreted TECK.
Horseradish peroxidase-conjugated streptavidin was used according to the manufacturer's instructions to detect the transferred DNA.
HC2 HPV test (Digene, Gaithersburg, MD) was performed according to the manufacturer's instructions to detect HPV, starting from 4 mL of liquid cytology specimens.
After the incubation cells were fixed and stained for EdU, according to the manufacturer's instructions to detect the number of cells in cycle during the EdU treatment.
In some experiments, the Annexin V-FITC Apoptosis Detection Kit (BD-Bioscience Pharmingen, San Diego, CA, USA) was used, according to the manufacturer's instructions, to detect apoptosis.
We used the ProSpect Rotavirus R240396 (Oxoid, Kamstrupvej, Denmark) enzyme immunoassay kit, according to the manufacturer's instructions, to detect group A human ROTAV in fecal specimens.
The Human cytokine LINCOplex KIT (13-plex) assay was used as per the manufacturer's instructions to detect a range of thirteen cytokines.
Secondary antibody was conjugated with horseradish peroxidase (anti-HRP conjugated rabbit antibody; Bio-Rad, Hercules, CA) and enhanced chemiluminescence lighting (Pierce Chemical Company, Rockford, IL) was used according to the manufacturer's instructions to detect salivary amylase.
