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Monocytes were purified using the CD14 positive mononuclear portion which was isolated according to manufacturer's instruction provided with the pan monocyte isolation kit.
For immune-precipitation analysis, whole-cell lysates or nuclear fractions were extracted following the manufacturer's instruction provided with the Universal Magnetic co-IP kit (54002, Active Motif).
Samples were prepared based on the instruction provided with the Annexin V Apoptosis Kit.
The diagnose instruction provided with the egenv tool helps to trouble-shoot and rectify problems if there are legitimate authentication issues.
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BMSCs were cultured to passage 3 and plated in 6-well plates for β-galactosidase staining following the manufacturer's instructions provided with the β-Galactosidase Staining Kit (Cell Signaling Technology, Danvers, MA).
Credit card payments should be made up to the limit established by the credit card bank, in accordance with specific instructions provided with the invoices, to the Lockbox Bank designated for credit card payments.
Serum testosterone level was assayed by ELISA technique using testosterone assay kit purchased from Kamiya Biomedical Co., according to the manufacturer's instructions provided with testosterone assay kit.
Total RNA was extracted according to instructions provided with the utilized Trizol reagent (Invitrogen, Carlsbad, CA, USA); RNA concentration and content was evaluated via spectrophotometer, and cDNA was synthesized by reverse transcription of the RNA.
Saliva can be collected from adults by following the user instructions provided with the Oragene DNA self-collection kit.
Chemiluminescence detection was performed according to the instructions provided with the Western Exposure Chemiluminescence Kit (GE Healthcare, USA).
DNA constructions were carried out by using established procedures [31] or following the instructions provided with specific reagents.
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