Sentence examples for instruction pierce from inspiring English sources

Nuclear extracts were prepared using NE-PER nuclear and cytoplasmic extraction reagents based on the manufacturer's instruction (Pierce, Rockford, IL, USA).

Cytoplasmic and nuclear protein fractions were prepared using NE-PER nuclear and cytoplasmic extraction reagents based on the manufacturer's instruction (Pierce, Rockford, IL).

The supernatant (whole cell lysate) was collected and the protein concentration of the lysates was determined by the bicinchonninic acid (BCA) protein assay according to the manufacturer's instruction (Pierce).

Specific proteins were visualized with enhanced chemiluminescence detection reagent according to the manufacturer's instructions (Pierce Biotechnology).

Target proteins were visualized with enhanced chemiluminescence detection reagent according to the manufacturer's instructions (Pierce Biotechnology, Rock-ford, IL, USA).

At this point, proteins were either directly stained in gels with silver nitrate, as per manufacturer's instructions (Pierce® Silver Stain, Thermo Scientific), or transferred to membranes for Western blotting with 17G1 as above.

Five micrograms of OsSMF1 protein were incubated with 40 fmol biotin-labeled double-stranded oligonucleotides, 1 μg poly dI-dC, 1X binding buffer, 2.5% (v/v) glycerol and 0.05% (wt/v) NP-40 in a 20-μl reaction volume for 1 h at room temperature, according to the manufacturer's instructions (Pierce).

Membrane/cytoplasm fractionations were done using Subcellular Protein Fractionation Kit according to the manufacturer's instructions (Pierce, ThermoScientific).

SsDPSL immobilization on the AminoLink Plus Coupling Gel (4% cross-linked beaded agarose, 50% slurry) was performed according to the manufacturer's instructions (Pierce; Rockford, IL).

Respective horseradish peroxidase (HRP) labeled secondary antibodies were applied and enhanced chemiluminescence (ECL) detection was used according to the manufacturer's instructions (Pierce, Rockford, IL).

Blots were detected using the Supersignal West Femto, according to the manufacturer's instructions (Pierce, Italy), and chemiluminescent signals were recorded with the ChemiDoc XRS imager Bio-RAD Laboratoriess). Bio-RAD Laboratories