Finally, those patients who were considered not able to cooperate formed a third group, group C, and the scan was performed without any respiratory instructions or with the simple instruction that the patient should take a "shallow breath".
Mucin was detected by staining with periodic acid-Schiff reagent (Sigma, St . Louis MO) as per manufacturer's instructions or with Alcian blue, pH 2.5 (Sigma).
adhesion assay Tissue culture plates (96-well) were pre-coated with bone marrow host cells to reach 70%% confluency or with nonspecific attachment factors (Chemicon) following manufacturers' instructions, or with human endothelial cells.
Complementary DNA (cDNA) was synthesised using the Fermentas Revert Aid H Minus First Strand cDNA Synthesis kit following the manufacturer's instructions, or with random hexamer primers and superscript II reverse transcriptase (Invitrogen, Carlsbad, CA, USA), according to the standard procedures.
Cells were either stained with β-galactosidase staining kit (Cell Signaling Technology, Beverly, MA, USA) according to the manufacturer's instructions or with C12FDG as previously described in Debacq-Chainiaux et al. Briefly, cells were pre-treated for 1 h with 100 nM bafalomycin A1 and further incubated for 2 h with 33 μM C12FDG (a fluorogenic substrate of β-galactosidase).
Reading in this way is a particular and necessary pleasure, quite different from reading for instruction, or identification with feeling.
Most of the research on biology students' attitudes relies on survey data, comparing student responses either before and after instruction or across courses with different classroom interventions, as discussed in Emerging Research on Biology Student Epistemologies.
On the flip side, if your partner initially agreed that you could give the children religious instruction or take them with you to church or temple, you have a right to insist on it.
We used an 'item centred' coding scheme based on Tourangeau's Cognitive Aspect of Survey Methodology Framework to identify 'problem' items (i.e. those with which participants had difficulty following instructions or problems with comprehensibility, acceptability, relevance or answerability) [ 14].
Equal volume of exosome preps from control, M. smegmatis and M. avium infected macrophages were resolved on 10% SDS-PAGE and either stained with SilverQuest silver-staining kit (Invitrogen, Carlsbad, CA, USA) according to manufacturer's instructions or immunoblotted with anti-Hsp70 antibody (Assay Designs, Ann Arbor, MI, USA).
