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Following the manufacturer's instruction of Mitochondrial ToxGlo Assay (Promega), the amount of ATP was measured by luminescence using a plate reader (Synergy HT, Biotek).
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WST-1 (Roche) assay measuring the activity of mitochondrial dehydrogenases was performed following the manufacturer's instruction at 0-, 1-, 2-, 3-, 4-, 5- day time points.
Detection of mitochondrial potential change was performed using JC1 (Sigma, CS0390) staining kit according to manufacturer' instructions.
Fractionation of mitochondrial proteins was done using a mitochondrial isolation kit (Sigma, St . Louis MO) according to manufacturer's instructions.
Avery was given a diagnosis of mitochondrial disorder at birth.
Aging is associated with declined mitochondrial energy metabolism, enhanced mitochondrial oxidative stress, increased production of mitochondrial reactive oxygen species (mtROS), and accumulated mitochondrial DNA mutations.
Additional analyses of mitochondrial membrane potential conduction.
As a function of mitochondrial biogenesis, ratios of mitochondrial to nuclear DNA were measured.
Dysregulation of mitochondrial genes and disruption of mitochondrial membrane potential in SBMA mice.
Akt mediates mitochondrial protection in cardiomyocytes through phosphorylation of mitochondrial hexokinase-II.
Herbert, M. & Turnbull, D. Mitochondrial replacement to prevent the transmission of mitochondrial DNA disease.
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