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This had two major new features that were to become computer standards: a two-level store and instruction modification registers (which soon evolved into index registers).
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(Neil) Whilst assessments would generally be delivered according to the standardised instructions, modifications to wording or particular item content were sometimes made in order to make the test material more relevant or localised to the immediate context.
The ability to follow instructions will be gauged by asking the patient a series of 1-step and 2-step instructions, a modification of the De Jonghe et al 6 approach which we have previously reported.
RNA was extracted according to manufacturer's instructions with modification for the small blood volumes (PaxGene Blood RNA kit, QIAGEN, Germany).
This assay was conducted according to manufacturer's instructions, with modification as follows.
EpiTYPER® CpG methylation profiling was done per manufacturer instruction, with modifications as described here.
The procedure was mainly based on the manufacturer's instructions with modification.
Hybridization to the BeadChip was performed according to the manufacturer's instructions without modification.
DNA was extracted using the QIAamp DNA Blood Kit (Qiagen, Courtaboeuf, France) according to manufacturer's instructions with modification.
The Cell Death Detection ELISA PLUS kit (Roche, Indianapolis, IN, Catalog Number: 11774425001) was used according to the manufacturer's instruction and modifications described previously [23].
ChIP assays were performed using a ChIP Assay Kit (Upstate Cell Signaling Solutions) according to the manufacturer's instructions with modification.
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