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First strand cDNA was synthesized from 1 μg of the re-purified total RNA using 1 μl of RT primer mix (provided with the QuantiTect reverse transcription kit, Cat. No. 205310, Qiagen) and 1 μl of SuperScript III reverse transcriptase (200 U/μl) in a 20-μl reaction according to the manufacturer's instruction (Life technologies).
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Of course, for the person on the receiving end of poor instructions life can be very frustrating.
Reverse transcription and pre-amplification were performed following the manufacturer's instructions (Life technologies, CA).
Training in the first cohort consisted mainly of lectures and video demonstrations, whereas the second training was much more structured with short instructions, life demonstration, and compulsory role-plays for the participants to train the main therapeutic techniques.
A week later the paper ran a response from the third grade at Nantucket Elementary School, which as it happened was fresh from special instruction in "life skills": "We read what you said, and we don't like it.
The Mathematics and Computer Science and the Biology Departments both offer courses that provide mathematical, statistical, and computational instruction for life science majors at Emory College.
In this way, Purnomo lived up to the simple philosophical instruction for life received from his father, Dulmuntalib Projodipuro: bring harm to no one; protect from harm as many people as possible.
DNA makes up genes, the instructions of life, while RNA conveys those instructions.
Then the nuclear DNA, which contains all the instructions for life, is transferred from the sick woman's egg.
That's true even after he develops an alter ego, François (also played by Mr. Cera), a French-accented sophisticate with a wispy mustache who proceeds to boss Nick around, giving him instructions on life and, of course, l'amour.
With instructions for life dished up with tough love, viewers could never mistake the Huxtables for the Brady Bunch.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com