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The instruction, "Please identify," was presented for 5 s before each trial block above an instruction image specifying the facial features for the targets in the following task block (Fig. 1).
Before each block, participants were presented with an instruction image indicating which task they would perform next, and which response the first response key indicated.
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To detect caspase-8 activity in situ in living microglia, a caspase-8 detection kit containing a specific and fluorescent FAM-LETD-fmk that covalently labels active caspase-8 was used according to the manufacturer's instruction (Image-iTs LIVE Green Caspase-8 Detection Kit, Invitrogen, Carlsbad, CA, USA).
Following kit instructions, images were captured using an Eclipse E400 microscope (Nikon) and 50 cells in random fields of view were quantified for blue coloration indicating senescence.
Table 3 Preferences for automated cars' programming instructions Open image in new window Table 4 Resposes to attitudinal questions Open image in new window.
Follow the same instructions from image 6 and apply it on the other eye.
In-gel protein staining by Sypro Ruby (Invitrogen) was conducted per the manufacturer's instructions, and imaged in a VersaDoc MP5000 imager (BioRad) with UV trans-illumination.
Some postal voting instructions contained images that appeared to favour one result over the other.
Western blots were performed using the LI-COR detection system according to the manufacturers instructions and imaged using a Odyssey Infrared Imaging System (LI-COR Biosciences).
Immunodetection was carried out using the ECL western blotting detection system (GE Healthcare) according to the manufacturer's instructions and images were obtained using BIOMAX™-XBT X-ray film (Kodak).
Quantification of the volume ratio between different cell populations in differentiated neurospheres was performed utilizing SlideBook4 software according to the manufacturer instructions (Intelligent Imaging Innovations, Denver CO).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com