(For information: www.birds.cornell.edu/pfw/.) A $15 fee includes instructions, a handbook about birds and bird feeding, a poster with color illustrations of common feeder birds, a calendar, data forms and return envelopes.
Total RNA was extracted from plant samples following the instructions in handbook of Trizol (Invitrogen) and treated with RNase-free DNase I (Promega) to remove contaminated DNA.
* Of course, many composition handbooks also give instruction and exercises (see Handbooks and Guides for Students).
A pair of bait and prey plasmids were transformed into the Y2HGold yeast strain following the Clontech Yeast Protocol Handbook instructions (Clontech, Mountain View, CA) and plated on minimal medium lacking Trp and Leu (SD/-Trp/-Leu) with X-α-Gal (the substrate of MEL-1 gene product, α-galactosidase).
The RNA was extracted using the RNeasy Mini kit, according to the manufacturer's instructions (RNeasy Midi Handbook; Qiagen, Germantown, MD, USA).
For testing APs interactions, combinations of APs-bait and APs-prey constructs co-expressed in yeast were subjected to α- and β-galactosidase assay according to the manufacturer׳s instructions (Yeast Protocols Handbook, PT3024-1, Clontech Laboratories , Inc.
This review was conducted in accordance with the Preferred Reporting Items for Systematic Reviews and Meta-Analyses PRISMA (Meta-Analyses PRISMAas well as following the instructions of Cochrane Handbook for SysteMoher Retiews of Interventions (Higgins ald Green, 2011).
For the CRC cohort and the methodological samples, RNA was purified using the miRNeasy FFPE Kit (Qiagen, Hilden, Germany) according to the manufacturer's instructions (miRNeasy FFPE Handbook September 2010, www.qiagen.com).
(And not without reason: the anarchist leader Johann Most, who spent time in New York, had published a pamphlet called "Revolutionary War Science: A Little Handbook of Instruction in the Use and Preparation of Nitroglycerin, Dynamite, etc.," which openly advocated the extermination of the middle classes).
