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The cell lysate was then centrifuged for 20 minutes at 35 000 g, the clarified supernatant was loaded onto a glutathione Sepharose 4B column (GE Healthcare) for 2 hours, and bound proteins were cleaved from the resin by incubating with PreScission Protease in accordance with the manufacturer's instruction for batch purification.
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mRNA enrichment of the samples was performed using Oligotex beads following the manufacturer's instructions for batch purifications (Qiagen).
All steps were performed according to the manufacturer's instructions for small scale batch mode expression (WEPRO1240H Expression Kit_E_ver. 2.0, Aug. 13, 2007) [ 42, 43].
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All supplies to be used in the study will be UK licensed stock prescribed by a dentist and labelled in accordance with current regulatory requirements for Prescription Only Medicines including instructions for use, expiry date and batch details.
Five replicates were detected for batch-to-batch analysis.
Julie has instructions for him.
(Instructions for creating both are at laurieberkner.com).
These are my instructions for tonight.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com