Sentence examples for instruction except the from inspiring English sources

The control group did not receive any treatment in the fifteen sessions of instruction except the traditional reading comprehension strategies such as skimming, scanning, doing inferences, reading for the main ideas, etc.

All experiments were performed according to the manufacturer's instruction except the X-Gal assay.

The protocol was adapted according to Müller HM. et al. [ 25]. Isolation was performed according to the manufacturer's instruction except the following protocol steps: the 800 μl of serum samples were split into 2 aliquots of 400 μl and each mixed with 400 μl of Roche Binding Buffer and 80 μl ProteinaseK (20 mg/μl Fermentas, St.Leon-Rot, Austria).

The cDNA libraries were constructed using the UNI-ZAP® XR Vector System (Stratagene, La Jolla, CA) following the manufacturer's instructions, excepting for the libraries L08 and L11, where the kit "Creator Smart cDNA library construction" (Clontech, Mountain View, CA) was used.

cDNA normalization was performed from total RNAs with MINT and TRIMMER kits from Evrogen according to the manufacturer's instruction, except that the number of PCR cycles for material amplification was adapted to our material.

Blood DNA was isolated from 200 μl heparinised blood sample using QIAamp DNA mini kit (Qiagen, Crawley, UK) according to the manufacturer's instruction, except that the DNA was eluted with 50 μl Buffer AE and incubated at 65°C for 5 minutes before centrifugation.

DNA was isolated from the culture using UltraClean™ BloodSpin™ Kit (MO BIO Laboratories, CA, USA) according to the manufacturer's instruction, except that the DNA was eluted with 100 μl Buffer 5 and incubated at 65°C for 5 min before centrifugation.

RNA was then purified using the RNeasy® kit (Qiagen) according to the manufacturer's instructions, except the RW1 buffer wash step was not performed.

Protein samples (1 5 μg) were run in 10 % NuPAGE as described in the manufacturers instructions except the reducing agent, 50 μM DTT, was omitted in non-reduced samples.

The Quantitect™ SYBR® Green RT-PCR kit (Qiagen) was used according to the manufacturer's instructions except the final PCR volume was reduced to 25 μl.

The RNeasy RNA Isolation Kit (Qiagen) was used to isolate RNA from each sample, following manufacturer's instructions except the final step where genomic DNA was removed by using the DNA-free Kit (Applied Biosystems) per manufacturer instructions.