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According to the manufacturer's instruction described in 'Materials and Methods', less than 25000 cells/40 μl/well were used in the 3D plates.
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Hence to avoid this, the instructions described in the SOP must be understood.
The students comprising the control group performed the experiments following the instructions described in the laboratory manual.
Therefore, we consequently intended to guide the manual defining of the crescent-shaped VOI to further reduce the inter-reader variability using the instructions described in the "Methods" section.
Given the possible benefits of using these games to the generalization of reading and writing skills, it is important to emphasize that, different from some of the online and board games available for purchase, these games can be designed by anyone who can follow the design instructions described in the methods section.
For this the Affymetrix Two-Cycle Target Labelling and Control kit was used (P/N 900494) according to the manufacturer's instructions described in the Affymetrix Eukaryotic Sample and Array Processing Manual version 701027 Rev.5.
After being reduced, alkylated, and digested with trypsin, the samples were labeled following the manufacturer's instructions described in the iTRAQ protocol.
Amplified cDNA was consequently purified by MinElute Reaction Cleanup Kit (QIAGEN Inc., Valencia, CA, USA) according to the instructions described in the WT-Ovation One Direct protocol.
The trademark indicates that the information has been collected with an official CQI patient survey, by a certified 'survey vendor' according to rules and instructions described in the CQI Manual [ 15].
The need of nursing instruction described patients' needs in order that they can understand the diseases and therapy procedures, effects and side effects of drugs, management of side effects, diet restrictions, significance of each test, daily care, and methods of emergency medical treatment.
Apoptotic cells were determined by terminal deoxynucleotidyl transferase biotin-d UTP nick end labeling (TUNEL) assay using a situ cell death detection kit (Roche Diagnostic, Indianpolis, IN) according to the manufacturer's instruction as described in our previous studies [19], [37], [47].
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