Contiguity (Moreno and Mayer 1999) and coherence (Mayer et al. 2001) limit the cognitive load of instruction by using visual information to support comprehension and restricting unnecessary information that would require the use of additional cognitive resources to inhibit (Pasolunghi et al. 1999).
Protein concentration was determined by use of the Bio-Rad Bradford Protein Assay (Bio-Rad Laboratories) according thethe manufacturer's instructions.
We specifically decided to have PhD-level instructors, because we wanted instructors with expertise in scientific thinking, but we hope that it is possible to replicate the same quality of high-level instruction by using more advanced graduate students or postdoctoral fellows.
The single-tube TaqMan miRNA assays (Assay Id 000413, Applied Biosystems, Life Technologies) was used to detect and quantify mature miR-29b according to the manufacturer's instructions, by the use of iQ5 multicolor detection system (Bio-Rad, Berkeley, CA, USA).
The Matrigel coverage was prepared according to the manufacturer's instructions by using 70 µl of Matrigel to cover an 8-well Lab Tek Permanox chamber slide (Nalge Nunc International).
Reverse transcription was performed by using iScript Advanced cDNA Synthesis Kit for RT-qPCR (BioRad) according to the manufacturer's instructions by using 5 μg of total RNA as template.
In this paper, it explains the purpose of, instructional manual for creation of, and usage instructions for video based analysis (VBA) by used of Window Live Movie Maker (WLMM), an emerging technique of video review attempting to encourage the use of video-based analysing teaching in PE.
A video tutorial and step-by-step instructions for use of PhenoMiner can be found on the RGD website (http://www.rgd.mcw.edu/wg/home/rgd_rat_community_videos/introduction-to-the-rgd-phenotypes-and-models-portal) and in a recent publication (7).
It provides an overview of the software and gives step-by-step instruction in the use of various features.
As a teenager, I was introduced to guns by instruction and my use of guns was restricted.
