Breast cancer cells were transfected with target and control siRNAs (50 nmol/L per well in 100 μL total volume) in 96-well plates, according to the manufacturers' instructions as previously described [ 44, 52].
Thrombin generation was measured with the thrombinoscope assay (Thrombinoscope BV, The Netherlands), according to the manufacturer's instructions, as has previously been described by our group [ 6, 7, 22].
The Infinium HumanMethylation450K BeadChip (Illumina) was used to analyze the genome-wide methylation profile of 122 gliomas according to the manufacturer's instructions, as previously described (Supplementary Table S1 31,32.
Luciferase assays were performed by the Luciferase assay system (Promega) according to the manufacturer's instructions as previously described [12].
8-Plex iTRAQ (Applied Biosystems) sample preparation and labeling were performed according to manufacturer's instructions as previously described [23] with samples from Experiment 1 (Table 1).
NK cells were depleted by i.v. injection with 50 µl anti-ASIALO GM1 polyclonal antibody (Wako Chemicals, Osaka, Japan) or rabbit serum on day 22, 26 and 30, according to manufacturer's instructions as previously described [37].
Human peripheral blood monocytes (PBM) were isolated by centrifugation through a Ficoll gradient followed by CD14-positive Magnet-Assisted Cell Sorting (MACS, Miltenyi Biotec, Auburn, CA) according to manufacturer instructions as previously described [11].
Multiplex fluorescence in situ hybridisation (M-FISH) analysis was performed on metaphase spreads prepared from Res259 cells using a Vysis SpectraVysion probe (Abbot Molecular, Abbott Park, IL, USA) following the manufacturer's instructions as previously described [15].
Released cytokines TNFα and IL-6 and chemokines CXCL10 (IP-10), MIP-2α and IL-8 were quantified by DuoSet ELISA (R&D Systems, Minneapolis, MN) following the manufacturer's instructions as previously described [19].
