Sentence examples for instruction and treated from inspiring English sources
Total RNA was extracted using TRIzol reagent (Invitrogen) according to the manufacture's instruction, and treated with RNase-free DNase-I (Takara, Otsu, Shiga, Japan) for removal of potentially contaminating DNA.
Total RNA was isolated from cardiac mouse tissue using the NucleoSpin RNA II kit (Macherey-Nagel), according to manufacturer's instruction and treated with DNase.
For quantitative real-time PCR, total RNA from cell lines was extracted with the RNAzol B (Biogenesis, Poole, UK) according to the manufacturer's instruction and treated with DNAse 1 (Invitrogen, Paisley, UK).
Total RNA was extracted from each sample using TRIzol Reagent (Invitrogen) or RNeasy Micro Kit (Qiagen) according to the manufacturer's instructions, and treated with 2 U DNase I (Ambion) for 30 min at 37 °C.
Total RNA was extracted from each sample using the Trizol reagents (Invitrogen) according to the manufacturer's instructions and treated with DNase I (Promega, Madison, WI).
For primary human breast carcinomas, total RNA was extracted using Trizol reagent (Life Technologies) following the manufacturer's instructions and treated with DNaseI (Qiagen).
Total RNA was isolated from the dissected tissues using TRIzol Reagent (Invitrogen) according to the manufacturer's instructions and treated with DNase I (RNase-Free) (Ambion).
Total RNA was extracted using the RNeasy Mini Kit according to the manufacturer's instructions and treated with RNase-free DNase I (Qiagen).
Total RNA from freshly isolated or magnetically sorted lymphocytes from each organ was isolated using TRIzol Reagent (Intron) according to the manufacturer's instructions and treated with DNaseI.
Samples were purified with the RNeasy Mini kit (Qiagen) according to the manufacturer's instructions and treated with DNase I (RNase-free DNase Set, Qiagen).
Total RNA was extracted from a pool of five placental samples coming from at least two litters from the same group with Trizol (Invitrogen Life Technology) according to the manufacturer's instructions and treated with DNase I to eliminate genomic DNA contamination.
