Sentence examples for instruction and subsequently from inspiring English sources
Prior to analysis, we formatted the discriminating gene expression values from the test datasets onto spreadsheets according to software instruction, and subsequently applied the data to the software.
And if the phone is being used for navigation instructions and subsequently loses its cellular connection, the turn-by-turn alerts will also disappear.
On 30 November, she rendezvoused with Karanj at sea to transfer instructions and subsequently then left for Bombay and reached there by 4 December 1971.
For co-immunofluorescence to identify activated caspase 3 – positive and TUNEL – positive cells, slides were first TUNEL stained according to manufacturer's instructions and subsequently blocked and assayed with the activated caspase 3 antibody as above.
and 7-actinomycinD (7-AAD; Invitrogen) according to the manufacturer's instructions, and subsequently analysed by flow cytometry on a four laser LSR II SORP flow cytometer (BD Biosciences, San Jose, CA).
On the following day, myoblast cells were transfected with vinculin expression constructs using Nanofectin transfection reagent (PAA) according to the manufacturer's instructions and subsequently handled as detailed for MEFs (see above).
RIP and input RNA was treated with DNase I (Life Technologies) according to the manufacturer's instructions and subsequently ethanol precipitated.
Total genomic DNA was extracted using a QIAGEN DNeasy Plant Mini Kit QIAGENN, Mississauga, Ontario, Canada) following manufacturer's instructions and subsequently stored at −20°C.
Total RNA from pooled tissue was extracted using Trizol reagent (Life Technologies) according to manufacturer's instructions, and subsequently column-purified with miRNeasy kits (Qiagen).
T cells were embedded in HistoGel (Thermoscientific, Cheshire, UK) according to the manufacturer's instructions and subsequently fixed with 4%% paraformaldehyde for 24 h.
Co-immunoprecipitation was performed using the Pierce HA Tag IP/Co-IP KiThermormo Fisher) according to the manufacturer's instructions and subsequently analyzed by western blot analysis.
