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Total RNA and DNA were extracted from cell lysates according to the manufacturer's instruction, and quantified spectrophotometrically.
Total RNA was isolated from liver samples with a "Total RNA" kit (A&A Biotechnology, Gdynia, Poland) according to the manufacturer's instruction and quantified with a NanoDrop 1000 (NanoDrop Technologies, Wilmington, Delaware, USA).
Biotinylated cRNA was normalized and hybridized to the Illumina Human HT-12V4 Expression BeadChips according to the manufacturer's instruction and quantified using an Illumina iScan System (illumina, San Diego, CA, USA).
Total RNA was isolated from 5-10 × 105 trypsinized USSCs and from mechanically dislodged pools of spheres using RNeasy Mini Kit (Qiagen, Germantown, MD, USA) and RNase-Free DNase I Set (Qiagen) according to the manufacturer's instruction, and quantified using a NanoDrop 1000 Spectrophotometer.
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In order to design a CPU which has such varieties in each of the different instructions, we need to go through each instruction and quantify what must happen during each cycle.
After indexing, the PCR products were purified using Agencourt AMPure XP – PCR purification beads (Beckman Coulter, Brea, CA, USA) according to the manufacturer's instructions and quantified using a dsDNA BR assay Kit (Invitrogen, Carlsbad, CA, USA) on a Qubit 2.0 fluorometer (Invitrogen, Carlsbad, CA, USA).
RNA was extracted according to the manufacturer's instructions and quantified by measuring absorbance at 260 nm.
Valencia, CA) following manufacturer's instructions and quantified by Nanodropspectrophotometric method. 1 µg of RNA was converted to cDNA using Verso cDNA kit (Thermoscientific).
Total RNA was isolated from about 100 mg tissue using TRIzol (Invitrogen, Carlsbad, CA), according to the manufacturer's instructions, and quantified by spectrophotometry.
RNA was isolated from the lysate supernatants using TRIzol® (Invitrogen) according to the manufacturer's instructions and quantified in triplicate by measuring absorbance at 260 nm.
RNA was extracted with the NucleoSpin RNA II kit (Macherey-Nagel, Hoerdt, France), according to manufacturer's instructions and quantified using Nanodrop (Thermo Fisher Scientific, Courtaboeuf, France).
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