Staining was performed at indicated time points after IR according to the manufacturer's instructions, and flow cytometry was conducted on a flow cytometer (BD FACScan).
Staining was performed according to the manufacturer's instructions, and flow cytometry was conducted on a FACSCalibur (Becton Dickinson, Mountain View, NJ, USA).
A discussion with the 40 schoolchildren on the draft Malay Child-OIDP index showed that the questionnaire was well understood in terms of its purpose, content, wording, general layout, instructions and flow with only minor modifications.
Apoptosis was evaluated using the Annexin-V-FLUOS Staining Kit (Roche Applied Science), according to the manufacturer's instructions, and flow cytometry was performed using a FACSscan (Becton and Dickinson).
Cell counts and phenotype was determined by BD Truecount tubes according to manufacturer's instruction and by flow cytometry.
The cartridges were washed according to the manufacturer's instructions and the flow through discarded.
Proteins BthTX-I in Fc (Flow cell, Fc = 1) and BthTX-II in (Fc = 2) were covalently immobilized on the Serie S GE-BIAcore CM-5 sensorchip; they have carboxymethylated dextran matrix, according to the manufacturer's instructions, and the flow cells Fc = 3 and Fc = 4 were the blank (negative control).
Lymphoma cells cultured with or without rhArg were labeled with JC-1 probe as manufacturer's instruction and analyzed by flow cytometry.
Subsequently, cells were processed using Click-iT EdU AssayFluor 647 Assay Kit (Life Technologies) according to the manufacturer's instruction, and subjected to flow cytometry.
These instructions disable DTR, RTS and flow control.
