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DNA fragments are separated using an ABI PRISM 3100 Genetic Analyzer (Applied Biosystems, Zug, Switzerland) according to manufacturer's instruction and analyzed with GeneMapper ID v3.2 software (Applied Biosystems, Zug, Switzerland), with minimum interpretation peak threshold of 50 relative fluorescence units (RFU).
Quantitative real-time RT-PCR was performed using SYBR-Green master mix (Applied Biosystems) according to the manufacturer's instruction and analyzed on an ABI 7300 Real-Time PCR system.
Lymphoma cells cultured with or without rhArg were labeled with JC-1 probe as manufacturer's instruction and analyzed by flow cytometry.
The PCR products were digested with restriction enzymes (NEB, UK) according to the manufacturer's instruction and analyzed by 2% (rs11889031, rs10932029, rs4675374, rs10183087) and 4% (rs10932037) agarose gel electrophoresis (Additional file 1, Figure S1).
Cytokines and chemokines were assayed using multiplex luminescent beads (Bio-Plex Pro Cytokine custom assay; Bio-Rad) according to the manufacturer's instructions and analyzed using a Bio-Plex Analyzer (Bio-Rad).
Cells were stained with the indicated antibodies (Additional file 2: Table S1) according to the manufacturer's instructions and analyzed using the MACSQuant™ Analyzer (Miltenyi Biotec) (Additional file 3: Figure S1).
The multiplex ligation-dependent probe amplification (MLPA) analysis was performed for BRCA1 and BRCA2 (SALSA MLPA kit P002-B1 for BRCA1 (lot 0508) and kit P090-A2 for BRCA2 (lot 0808), MRC-Holland, Amsterdam, the Netherlands) according to manufacturer's instructions and analyzed with ABIPRISM 3130xl Genetic Analyzer and Genemapper® v.4.0 software (Applied Biosystems, Foster City, CA, USA).
Appropriate test kits came from Seahorse Biosciences, were performed according to the manufacturer' instructions, and analyzed on a Seahorse 24XFe extracellular flux analyzer and Wave software (www.seahorsebio.com) as described elsewhere [ 31] and in detail in the supplemental information.
according manufacturer's instructions and analyzed by light microscopy.
Samples were genotyped using the manufacturer's instructions and analyzed on an ABI PRISM 7900 HT system (Applied Biosystems).
Samples were prepared according to manufacturer's instructions and analyzed on a BDFacsArray equipped with FCAP software (BDBiosciences).
