For the isolation and collection of surface proteins, we used the Pierce Cell Surface Protein Isolation Kit (ThermoScientific, Waltham, MA, USA), following the manufacturer's instructions adjusted according to the protocol described by De Wit et al (2012); Supplementary File S1 provides an elaborate description of the cell surface protein isolation and mass spectrometry protocol.
Medium 199 containing Earle's salt and HEPES (SIGMA, USA) was prepared from a powder according the manufacturer instruction (pH adjusted to 7.2 with NaOH) and sterilized by filtration.
Although this type of ambiguity was only rarely encountered in the data, it is possible that such an open-ended item could stimulate a response that gives a better indication of students' understanding if the instruction is adjusted to specify another audience, for example, "other students that have not taken the same course, but have a basic scientific background".
The proposed research provides the instruction to adjust surface panels efficiently and accurately.
The teachers again utilized their growing understandings of STEM-focused, project-based curriculum and instruction to adjust their collective approach.
This information can then be used to modify instruction accordingly, adjusting factors such as when and how to introduce new facts and visual details in a multimedia lesson.
The reactions were performed using the manufacturer's instruction after adjusting the reaction volume as 25 µl.
Refer to the operating instructions included with the laser printer for detailed instructions on adjusting the paper settings.
Three years later, the same court that disqualified the judge, the United States Court of Appeals for the Second Circuit, returned the case to him with instructions to adjust the amount of money.
If, in the opinion of the Judges this principle has been flouted, they can declare any entries or votes invalid, void the competition, change the competition instructions, or adjust the entries or the voting to achieve fairness, as they think fit.
