Sentence examples for instruction active from inspiring English sources

Cell fractionation was performed using the nuclear extract kit according to the manufacturer's instruction (Active Motif, Carisbad, CA, USA).

The nuclear and cytoplasmic extracts from bone marrow-derived macrophages were prepared, as per the manufacturer's instruction (Active Motif, Carlsbad, CA, USA).

RUNX-2 and PPARγ transcription factor activity was determined using a commercial TransAM™ assay as per manufacturers instructions (Active Motif, Belgium).

Antibody supershift reactions were performed following manufacturers instructions (Active Motif, Carlsbad, CA).

Nuclear extracts was prepared with a Nuclear Extract Kit according to manufacturer instructions (Active Motif, Carlsbad, CA).

To quantify active Runx2 binding, 15 20 mg of nuclear extract was measured using the Trans-AM Runx2 Kit according to the manufacturer's instructions (Active Motif, Carlsbad, CA, USA).

Nuclear extracts from the cells were prepared and p65 DNA binding activity was measured using the TransAM™ NFκB kit according to the manufacturer's instructions (Active Motif, Carlsbad, CA).

ChIP assays were performed according to the manufacturer's instructions (Active Motif, USA).

Nuclear extracts were prepared from C2C12/Runx2Dox cells treated with Dox according the manufacturer's instructions (Active Motif, Carlsbad, CA, USA).

NF-κB activation was assessed using the TransAM NF-κB kit according to the manufacturer's instructions (Active Motif, Belgium).

Nuclear extracts were prepared using a nuclear isolation kit and according to the manufacturer's instructions (Active Motif).