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We are even instructed to rinse some garbage.
Participants were instructed to rinse with distilled water at least four times over a 1 min period prior to testing.
The patients were instructed to rinse with a solution containing fuccina (Biodinâmica Química e Farmacêutica LTDA, Ibiporã, Brazil) for better identification of residual composite adhered to the tooth.
Subjects were randomly assigned 1 1 to either topical dexamethasone (.5 mg/mL) or tacrolimus (.5 mg/mL) solution and instructed to rinse with 5 mL for 5 minutes, 4 times a day, for 4 weeks.
Starting the second day after surgery, the patient was instructed to rinse twice daily with 0.5 oz. of chlorhexidine gluconate for 30 s after oral hygiene, and the patient was seen at least once 7 days after each surgical procedure for postoperative care and oral hygiene instruction.
All participants were instructed to rinse their mouth with tap water before collection.
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Before replacing in the mouth, therefore, patients were instructed to thoroughly rinse their aligners under running water to remove any trace of detergent.
After informed and written consent was obtained, the study subjects were instructed to provide rinse of oral cavity as we have previously described elsewhere [ 9, 17].
Mothers will be instructed to first rinse with 100 ml of distilled water and the collection is made by scraping the inner face of the cheeks with sterile cytological brushes, with circular movements repeated about 30 times.
After each inhalation, participants will be instructed to blow their nose, rinse their mouth and swallow the water to minimise postnasal drip and squamous epithelial cell contamination, respectively, before trying to expectorate in a sterile container.
The tattoo parlor operator was instructed to use only sterile water for rinse solutions and dilution of tattoo dye.
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