So far, the challenges of being the first woman to run the French capital have included defying the French language police to be called Madame La Maire (and not the grammatically correct masculine "le Maire") and being instructed by the protocol police not to wear trousers to meet the Queen on a recent state visit.
When bouncers were instructed about research protocol, aggression was defined as any verbal, physical or sexual behaviour that threatens the safety of the victim.
Briefly, one microgram of genomic DNA was first mixed with 5 µl of M-Dilution Buffer and incubated at 37°C for 15 minutes and then mixed with 100 µl of CT Conversion Reagent prepared as instructed in the protocol.
The protocol instructed women to weigh themselves in the morning before eating, drinking and dressing.
After labeling tumor DNA and reference DNA samples were pooled, cleaned and hybridization cocktails were prepared as instructed in the protocol.
The manufacturer's protocol instructed that an increase in signal greater than 40 units be taken to indicate an invalid test; however, such readings were not observed during this study.
For those proliferating cells, 5-bromo-2′-deoxyuridine (BrdU: Life Technologies, Grand Island, NY, USA) was incorporated with cells for 2 hours and detected using anti-BrdU antibody as instructed by company protocol.
Our protocol instructed that all participants would receive 2 g of IV cefazolin pre-operatively within 60 minutes of their procedures, and 2 g of IV cefazolin every three to four hours intra-operatively.
Fifteen 10 µm thick sections per biopsy were cut and collected in the same tube and their DNA extracted as instructed by the protocol for fixed tissues found in the manual of the QIAamp DNA mini Kit (Qiagen Inc., Valencia, California).
KW participated in developing the protocol, instructing the physical therapy staff on the treatment protocol, patient recruitment, research testing, contributed to trial registration on ClinicalTrials.gov, and drafted and critically revised this manuscript for important intellectual content.
