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For the EST data, we used transcripts per million (TPM) instead of absolute expression abundance, and for Q_PCR results, we calculated fold changes directly, according to the manufacture's instruction.
Therefore we use a normalized tag per million (TPM) [ 14] per base threshold and omit clusters with < 0.1 TPM per base instead of the total tag count.
The log-ratio (coefficients) outputs from limma package was used as adjusted log2 fold-change) [log2 fold-changeported instead of simple log2(fold-change) calculated from raw TPM values (differences between coefficients and raw TPM fold-change values are due to weights assignment to each observation calculated by the voom package).
Safety is a cornerstone of TPM.
The pharmacokinetics of TPM in plasma was linear, but TPM clearance from whole blood increased with increasing dose.
Therefore, a combination of TPM and percolation theory can bring us more precise information on ionic transport in SPPO matrices.
The raw number was normalized to a metric of TPM.
Accordingly, a total of 44 patients (8 men and 36 women) were randomized to receive the combination of TPM and NTP (23 received NTP and 21 received TPM).
Open image in new window Figure 2 Stretched diagram of TPM.
The TPM value (visualization) and the fold value (visualization) provide a graphic depiction of TPM and fold values as shown in Figures 2A and 2B, respectively.
Y-axis is RNA-seq expression level in natural log of TPM units (ln TPM)).
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