A number of lncRNAs have been reported to control transcriptional alteration, implying that the difference of lncRNA profiling between normal and cancer cells may have regulatory roles in cancer progression instead of being the secondary effect of cancer transformation [ 24].
Instead, most of the secondary cells are directly removed by Ubx-mediated PCD (Marin et al., 2012).
For samples co-stained with GARP and IL-1R2, a directly conjugated secondary anti-mouse IgG (BD Pharmingen) was used after GARP instead of the biotinylated secondary antibody.
Moreover, histopathology of the alveolar septae showed that instead of the elongated secondary septa seen in control lungs, SS-exposed lungs had an increased presence of crested secondary septae, indicating incomplete formation of secondary alveolar septa a feature also seen in human BPD (Ahlfeld and Conway 2012).
In the first step we use a smaller model architecture based on the {E,H,L} alphabet instead of the DSSP secondary structure alphabet.
Dylight 405 was used instead of the above secondary antibody to avoid the RPE autofluorescence without Sudan Black application.
Real Envision Detection system (DAKO, Denmark) was used instead of the traditional secondary antibody, sections were visualized with the chromogen DAB and counterstained with hematoxylin.
Instead of the use of secondary antibodies we have attempted monitoring these structural changes by an array of de novo designed synthetic and structured peptides.
The proposed method also solves Problem 1 directly, while (M2) and (M3) are indirect methods for the problem, because they predict common secondary structures instead of secondary structures of the target sequence.
To confirm that fluorine is substituted in the bulk disordered lattice instead of forming secondary phases at the surface of the particles, we first performed energy dispersive spectroscopy using a transmission electron microscope (TEM-EDS) (Fig. 2b).
