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"Individuals will call us to say they're ordering a book," he said, "and they say, 'I need it by this date because it is due for book club.' " Mr. Alexander said there are some cases where reading actually is verboten, when the entire group listens instead of reads, but those are rare.
Using seeds instead of reads, the seeds graph construction discards long-range continuity information in reads.
For better results, contigs after assembly, instead of reads, are used for annotation [ 21].
Considering tags instead of reads allows a high loss-less compression of the original data.
Once read classes are constructed, we only need a small modification of the E-step of IsoEM to use read classes instead of reads.
Overall our data showed that PCR amplification can be highly stochastic and non-uniform, and counting molecular barcodes instead of reads can efficiently remove PCR amplification variation.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com