These mutations can be classified based on whether they lie within the NRR core (class 1) or consist instead of insertions of 12 or more amino acids in the juxtamembrane regions immediately adjacent to the S2 site (class 2).
Thus, the experimental phases provided an unbiased map that suggested that instead of an insertion of a domain at residue 915, the metavinculin insert replaces vinculin α-helix H1 with α-helix H1' from the insert.
Thus, the carbonaceous deposition during the ethylene decomposition appears to take place only on the catalyst surface instead of lattice insertion.
Due to the insertion of 21 (class A) or 7 (class C) amino acid residues between the A and B parts of the HR-A/B regions, the class A and class C Hsfs have longer HR-A/B regions than class B, which is distinguished from class A and C by the presence of a heptad repeat pattern instead of an insertion.
However, a model using all windows with indels (instead of treating insertions and deletions separately), was not significant (data not shown).
However, instead of calling insertions based on one outgroup alignment only, PAGAN assigns weights to the graph edges either skipping a deletion or connecting an insertion to the rest of the graph and adjusts them according to the evolutionary evidence.
Instead, hotspots of insertions occurring in a single locus within few nucleotides of distance were observed (see above).
Additionally, utilizing the report of characteristic frequency of auditory neurons instead of percentage of insertion depth allowed the use of a semi-logarithmic scale, which is more relevant for clinical application than relative depth of insertion.
Instead, this subset of insertion sites had errors in locus-specific primer design due to incomplete or low-quality genomic sequences.
