Sentence examples for instantaneously frozen from inspiring English sources
From each individual used for qRT-PCR, the whiskers pad, the trigeminal ganglion and the brain stem were dissected from the ipsilateral side, instantaneously frozen on dry ice and kept at −80°C until further processing.
Heuser and Reese improved the temporal resolution of their analysis by building a 'freeze slammer' capable of instantaneously freezing a sample at arbitrary time points after stimulation (Heuser et al., 1979).
In the SFL process, a feed solution containing the API was atomized beneath the surface of a cryogenic liquid such that the liquid-liquid impingement between the feed and cryogenic liquids resulted in intense atomization into microdroplets, which were frozen instantaneously into microparticles.
Tissue samples were quickly excised, frozen instantaneously in liquid nitrogen and stored at −80°C until further processing.
Right lungs from some animals were frozen instantaneously in liquid nitrogen and stored at –80 °C for Western blot analysis and quantitative polymerase chain reaction (qPCR) for total RNA.
The brains were post-fixed overnight in the same fixative, cryoprotected in 20% sucrose in PBS for 48 h, then frozen instantaneously in isopentane at -45°C.
C-labeled biomass was harvested by centrifugation at 4000 rpm and 0° for 5 min. After centrifugation, the supernatant was poured off, and the cell pellet was frozen instantaneously in liquid nitrogen and stored at –80°.
Freeze until fully frozen.
This was achieved with a technique called 'high pressure freezing' that combines jets of liquid nitrogen with very high pressures to instantaneously preserve small samples without causing damage through the formation of ice crystals, or any shrinkage and distortion.
