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First, the paxilline-resistant current showed a nonlinear instantaneous rectification behavior similar to that of Slo3 (or Slo3+β4) current (Fig. 5C), while the Ca2+-dependent current exhibited linear instantaneous current behavior similar to Slo1 (or Slo1+β4) current (Fig. 5A).
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Thus, the instantaneous outward rectification of mSlo1+hβ2 should be the intrinsic characteristic of channels derived from the extracellular loop of hβ2.
The single-channel I/V curve of the mutant I323T displayed an outward rectification similar to the instantaneous I/V curve of the mutant I323T, which was clearly different from mSlo (Fig. 1, B2 and C2 ).
Normalized instantaneous I-V curves were used to determine the property of rectification of mSlo1+hβ2 channels.
Instantaneous currents showed that all of them can partially reduce the outward rectification ratio (Fig. 2B).
Additionally, the β2 like the β3b subunits possesses a nonlinearity on instantaneous current-voltage (I-V) curve of the resulting BK currents, termed rectification [16].
Unlike the instantaneous outward-rectified tail current of the α + h β2 or α + h β3 channels, the rectification of the mutation I323T does not come from the extracellular segments of the β-subunits (22).
"We called it our rectification.
The present is necessarily beyond rectification.
Maybe some sort of rectification was possible.
They were instantaneous friends, and instantaneous troublemakers".
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