For instance, probing the immunoblots with anti-phospho ERK1/2 antibody which only recognizes the dually phosphorylated form revealed that in response to heat shock at 43°C, the levels of phosphorylated EhMAPK increased as early as 15 min of exposure and became saturated at 60 min exposure (Fig. 5a).
For instance, probes corresponding to gains (which on average have higher intensities) are generally 'over-normalized' making a proper assessment of gains more difficult.
One potential problem is model overfitting since there were only a small number of positive instances (probe sets of known tissue-specific genes) available for this study.
Other rare examples are probes mapping across three exons where the central exon is generally a short exon, for instance the probe 207136_at:1089:1005 from the array HG_U133_Plus_2 maps to the transcript ENST00000307959 on three exons, 7 bp on ENSE00001275564, 10 bp on ENSE00001425447, and 8 bp on ENSE00001275540.
For instance, a probe for the TP63 gene (Tumor protein p63, keratinocyte transcription factor KET), AA455929, is ranked in the third position.
For instance, the probe density of CBA51/AuNPs was even higher than that of CBA45/AuNPs.
For instance, a probe may have been designed using proprietary transcript sequences or map to an unexplored area where tiling arrays indicate transcriptional activity [ 18, 19].
For instance, 3p14 probe loss was observed in 33.3% of CIN2 samples and 30.0% of CIN3 samples, whereas 89.5% of SCC samples displayed a 3p14 deletion.
There is much more to come, for instance the probes into interest rate and foreign exchange market manipulation.
Also, since the infants were measured at home throughout the night, there were instances of probe loss due to defecation and mothers were instructed not to replace the probe if lost to prevent injury of the child.
