Importantly, however, tandem BHT displayed no cooperativity during unfolding; taken together with the poor structural content revealed in the far-UV CD spectrum and some non-canonical gel filtration behavior seen in the presence of denaturants, this suggests a partially unsuccessful instance of protein design.
The general formulation followed herein for the description of each gene is presented in equation (1) below:
Another issue concerns the relevance of data produced by using high-throughput technologies, which represent a useful source of information and, therefore, are essential in a data integration approach: this is the case, for instance, of protein-protein interactions (PPIs) data, that enable the study of cellular networks structure by means of graph theory approaches.
It has previously been shown that functional instances of protein motifs tend to lie in intrinsically disordered regions of protein sequence [12], [13], [14].
'Chaining' refers to instances of protein domain sequences in a superfamily that cluster in an unexpected way.
All instances of protein absence were confirmed using TBLASTN against the genome with additional searches using sequences from closely related organisms.
Support vector machines (SVMs) have been trained with the feature-encoded data instances of protein stability changes upon amino acid substitutions.
In addition to object properties connecting instances of concepts, datatype properties are also used to associate data values with such instantances, e.g. hasSequence and hasWildtypeResidue associate string values with instances of Protein and PointMutation respectively.
We identified 65 instances of protein isoform pairs (See additional file 2: Additional File 2 for the list of these protein isoform pairs) wherein a domain alternates with another.
Only for BioPAX, it is important to use the appropriate classes (instances of smallMolecule for small molecules and instances of protein for proteins) and a nice feature to fill the available BioPAX fields for chemical formula or molecular weight of small molecules (SM annotations).
There are, however, several instances of protein pairs that contact in the 50S subunit for which no covariance is observed; clearly not every interaction will be identified by the sum of the coupling strengths, for example between two proteins that are held together primarily by the ribosomal RNA.
