The other instances of alternative splicing observed were: 8 exon skipping, 6 alternate acceptors, and 5 alternate donors.
In this study, we determined partial cDNA sequences for 88 open reading frames surrounding 246 introns in P. falciparum which were transcribed at schizont and gametocyte stages, and observed 15 instances of alternative splicing within a total of 14 gene transcripts, 16% of the analyzed genes.
Indeed, other instances of alternative splicing that produce viable protein resulting from the excision of entire domains have been reported, such as the vasopressin receptor [61].
Rare instances of alternative splicing and uses of the non-standard amino acid selenocysteine were also identified.
RNA-seq provides an efficient opportunity to investigate alternative splicing, which has revealed numerous instances of alternative splicing in mammals and plants [ 32, 41, 42].
Ebbole et al.'s [ 16] analysis of M. grisea ESTs led to the discovery of 134 instances of alternative splicing among 8,177 unique expressed sequences (1.6 %).
The examination of expressed sequence tag (EST) libraries from several M. grisea strains identified 134 instances of alternative splicing among 8,177 unique expressed sequences (1.6 %).
There were 1,069 instances of alternative splicing events that were restricted to a single tissue, most of them in testes (416) and brain (172).
In order to prevent this loss, contigs derived from 3' in-house sequences were examined, using a custom computational pipeline, for potential instances of alternative polyadenylation.
The human-mouse-dog comparison did not allow us to distinguish between true genome-specific, recently gained instances of alternative splicing and errors and artifacts.
Gene isoforms derived from alternative splicing are a significant contributor to extra-genomic variation [ 31] and exon-exon junctions have been used to identify instances of alternative splicing [ 32, 33].
