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All roots that would not pass through the sieve as well as roots that were easily removed by visual inspection were collected from each soil sample, briefly rinsed in DI to remove soil, dried at 60°C, and weighed to estimate BGB within each plot.
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The samples originated from skin test positive animals identified within the national or regional eradication programs, from abattoir surveillance, and from postmortem inspections of wildlife, and were collected from 1992 through June 2009 in different geographic areas in Spain.
Mandibular lymph nodes (LNs), tonsils, tracheobronchial LN, mediastinal LN, lungs, spleen, and mesenteric LN, as well as other affected organs were collected during field inspection and then subjected to detailed inspection in the laboratory and stored at −20°C.
The gene families were collected by visual inspection using CIRCOS software [ 75] (http://www.circos.ca).
All of the food-origin strains were collected by different Entry Exit Inspection and Quarantine Bureaus of China from 2005 to 2010.
Liver samples were collected during post-mortem inspection.
Outbreaks were typically reported by the municipal health service or the food inspection services to the RIVM, and samples were collected in close collaboration with these agencies.
Human population estimates were derived from the most recent census report, while pig population data were collected during American Samoa Environmental Protection Agency household inspections from June to October, 2006.
After turnout, the lambs were collected every fourteen days for inspection and treatment.
The remaining control samples were collected by the studbook during inspections, where paternal half-sibs of cases were sought.
The blood samples were collected along with the regular quarantine inspection of the farms, so no ethical approval was required for this study.
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