Tumor numbers were obtained by visual inspection of tissues in mice euthanized 21 days post-transplantation, and micrometastases were counted by pathologist's evaluation after dissection of the lung [7], [21], [56].
No complication, death, or sign of infection or tissue damage was noted by endoscopic observation or inspection of excised tissue at up to 12 weeks' follow-up.
2) Inspection of periodontal tissue around the tooth under investigation We measured the items shown in Table 4 at 6 positions (mesiobuccal, buccal, distobuccal, mesiolingual, lingual and distolingual) around each tooth under investigation.
On visual microscopic inspection of these tissue specimens, erlotinib-treated PC9 and HCC827 xenografts but not H1975 tumors exhibited a substantial reduction in Ki-67 positive cells as compared to the vehicle-treated controls (Fig. 3A and data not shown).
Ex-vivo lamb legs were used in this study because they represented a reasonable model without resorting to in-vivo animal models and allowed for macroscopic inspection of sliced tissues after exposure.
Importantly, inspection of the tissue-specific targets revealed no functional pathway enrichment (Figure S4).
Culture inspection of the resected tissue adjacent to the cavity detected E. faecium.
