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Inspection of sequences with incorrect sequence tags showed that the single largest contributor to the difference between the number of reads with proper primer sequences and the number of reads with proper sequence tag sequences was an N-1 truncation of the sequence tag, which presumably arose during the primer synthesis.
Inspection of sequences that are poorly conserved may also shed light on aspects of QSOX function.
Inspection of sequences flanking this gene did not reveal additional A, T, or C domains.
Inspection of sequences matching intergenic regions showed that most of them arise from telomeric or centromeric regions.
Indeed, visual inspection of sequences showed that most indels were actually located in homopolymeric tracts, where pyrosequencing is known to introduce errors [ 44].
However, after inspection of sequences immediately upstream and downstream of the different LiSIDER2s in chromosome 32, we did not find clear TSD sequences, even though when members of a subfamily were separately analyzed.
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Inspection of sequence around these lesions indicated that all four were due to homopolymer sequencing errors.
The inspection of sequencing electropherograms suggested the co-amplification of the COI locus from two different vertebrate hosts.
The inspection of sequencing electropherograms supported the amplification of the mitochondrial barcode locus from one single vertebrate host in the vast majority of cases.
Inspection of sequence around the remaining three candidates with a 0 score using CoGe's "Show" function and raw sequence data revealed that the putative yfaD polymorphism was due to a sequencing error and hence could be discounted.
To reconstruct the open reading frame of all the five MT1 pseudogenes, the corresponding genomic sequences were aligned with each of the MT1E exons separately, followed by manual inspection of sequence homology (Fig. 5).
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CEO of Professional Science Editing for Scientists @ prosciediting.com