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The RPE cells grown on scaffolds were examined by immunocytochemistry (ICC), MTT and continuous inspection of cellular morphology.
Furthermore, in the regression analysis, visual inspection of cellular staining patterns (Figure 8) also confirmed previous machine vision results [28] in terms of the ability of aldehyde and pyridinium or quinolinium building blocks to exert a predictive, additive contribution to diffuse vs. organelle-associated staining patterns, apparent across large numbers of images.
Mechanical micromanipulation is a classic method to isolate uncultivated microorganisms or early embryos, and it involves using a capillary pipette to suck up a single cell from a cell suspension with visual inspection of cellular morphology and coloring characteristics under a microscope [13, 14] (Fig. 1b).
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These samples were also sectioned to allow inspection of the cellular specificity of hybridisation.
Inspection of these cellular masses reveals the presence of a plethora of organised structures, including teeth, fingers and hair, suggesting the presence of pluripotent cells within the tumour.
Review of Cellular Metabolism.
Inspection of time course profiles in cellular content of Nrf2, Keap1 and NQO1 protein (Fig. 4G I) and statistical testing (α=0.95, autocorrelation function analysis) show that variation in response is that expected for white noise, implying that the time series are not oscillatory.
This proposal of cellular redistribution is supported by inspection of the arrangements of osx:eGFP-expressing cells, clearly much more selective for active bone-secreting osteoblasts (Figure 7).
Block-face observation using a scanning electron microscope (SEM) has also been recently applied to 3D observation of cellular components, but this is a destructive inspection and does not allow re-examination.
On histopathological inspection, large clear vacuoles, indicative of lipid droplets without evidence of cellular degeneration, were present in the proximal tubular cell cytoplasm in all mice, albeit more prominently in the mice that received rice bran.
Clearly, single-cell inspection, as recently shown for pancreatic progenitors57, provides a deeper understanding of cellular heterogeneity.
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