Before inspection, cells were grown for 2 days on solid minimal medium containing oleic acid as a sole carbon source.
Detailed inspection of cell cycle markers, rod and cone photoreceptor markers and immune cell markers suggested that Ward's retinoblastoma cluster 3 could reflect samples that contained a large proportion of normal-retina cells (Fig. S5D).
Because visual inspection of cell cultures treated with concentrations higher than 1.0 µM revealed increased cell death, most likely due to toxic side effects, we chose to test the effect of the three WNT inhibitors at a dose range from 0.1 µM to 1.0 µM.
The in vitro cartilaginous engineered constructs were evaluated by gross inspection, histology, cell proliferation, gene expression and sulphated glycosaminoglycan (sGAG) production at week 1, 2 and 3.
These methods, however, are based on microscopic inspection and cell counting by different researchers and, as a consequence, they are fastidious, time-consuming and error-prone.
Spots were filtered, confirmed and edited by visual inspection, and cell counts transferred to Prism (GraphPad Software, La Jolla, CA, USA) for statistical analyses.
Even after careful inspection of cell migration, axon guidance and synapse localization, we could not detect any major defects in the neurodevelopmental decisions made by AIY with these alleles (supplementary material Fig. S1; n>40 animals for each examined allele).
Inspection of cell means in Table 1 shows that the larger difference after size ratings mainly reflects particularly poor discrimination of benign images from pictures that had been rated for size (mean d' = 1.40, relative to the other three sensitivity means of 1.96, 2.03 and 1.82).
Microscopic inspection of cells grown under such conditions reveals that nearly the entire cell is filled with lipid particles [15].
Visual inspection of cells incubated with high-concentration CB nanoparticles showed the particles coating the cell surface (data not shown).
