Exact(3)
Inspecting the sequence of the cab12 promoter for the ACGT core sequence of bZIP factor binding sites reveals 12 positions for this motif (data not shown).
In inspecting the sequence alignment for the PurP-like proteins in both clusters, we found 100% conservation of Arg 314 and His 27 (M. jannaschii numbering), which make contacts to the formyl group in the MJ0136 crystal structure [ 66].
Since the RefSeq annotations of chicken and zebrafish are incomplete, we identified additional orthologous exon pairs by projecting the human annotation on the other species' genome and confirming the orthology of the projected exons by inspecting the sequence alignment of the projected exons in the representative species.
Similar(5)
We have manually inspected the sequence alignment of the homologous flanking ±100-bp regions.
We therefore inspected the sequence alignments of NA12716 and NA12760 in this region (SNVs 227 to 277; additional file 17).
Therefore, we inspected the sequence alignment and visually split all control region sequences into a 5' "variable" section (the first 39 - 49 bp) and a 3' "conserved" section (the remaining 493 base pairs).
Looking at the highly thermostable oxidase HotAldO for inspiration, we inspected the sequence alignment of AldO and HotAldO (Fig. 1) and identified the HotAldO residues that corresponded to the AldO residues with high B-factors.
To test the reliability of these called differences, we inspected the sequence reads of 307 selected genes (exhibiting in total 647 non-synonymous variants) using the Integrative Genomics Viewer (IGV) [ 42].
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