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The ECG tracings were manually inspected to correct for mislabeled beats.
The identified proteins are contrasted by computational analysis and their sequences are inspected to correct possible errors in function prediction.
All assembled contigs were also manually inspected to correct for errors due to duplicated segments and repeats; with special attention paid to regions having higher than 97% cross_match (v 1.080812) [ 54] identity.
The peptides released into the incubation buffer (the "surfome" or surface proteome) are analysed by LC/MS/MS, the identified proteins are validated by computational analysis and their sequences are inspected to correct possible errors in function prediction.
Chromatographic peak areas for the formaldehyde isotopologues (light, medium and heavy) of each detected peptide were first extracted using MSQuant [23] and then manually inspected to correct errors from overlapping peaks, missed monoisotopic peaks, etc.
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In the second annotation step, all predicted ORFs were manually re-inspected to correct start codon and function assignments.
In a second annotation step, all predicted ORFs were manually re-inspected to correct start codon and function assignments.
All integrated peaks were manually inspected to ensure correct peak detection and accurate integration.
All aligned sequences were then inspected manually to correct for apparent mistakes.
Selected full-length H1 HA sequences were downloaded from Genbank and aligned using Clustal W. All aligned sequences were then inspected manually to correct for apparent mistakes.
Trees were inspected manually to correct or refine the core/shell classification and to determine the origin of the transfers.
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