(8) Assign cell clusters to putative cell types based on inspecting the expression of known cell type markers and the identified signature genes.
To examine this possibility, reverse transcriptase PCR (RT-PCR) was used to inspect the expression of all members of the DCL, AGO, RDR and Double-stranded RNA binding protein (DRB) genes.
To examine which of the miRNA family members are more abundantly expressed in rice seedlings, we inspected the expression profile of known miRNAs in untreated seedlings (control library) based on their sequence frequencies.
We then inspected the expression levels of the A-IPACs and their respective targets to provide possible mechanisms of gene expression regulation.
First, we inspected the expression profiles of the Vitis vinifera stilbene synthase gene family [ 19] throughout our experimental design.
We inspected the expression patterns of SMB on GENESTIGATOR [ 67] but failed in an attempt to find any clues for their diversified roles in secondary wall formation.
In order to inspect the expression pattern of tRFs in abiotic stress treatments, we conducted an analysis of the AlaAGC, ArgCCT, ArgTCG and GlyTCC 5' tRFs, using the available deep sequencing data.
Inspecting the expression profiles of Ctnnb1 and its associated proteasome related genes shows a different expression in the LF compared to the HF group at t = 48, so this mechanism could be affected by diet in the late response to the glucose challenge.
Additionally, we inspected the gene expression of CYP6E14 and CYP6AE11 (with 91% nucleotide identity to CYP6E14) across all gossypol concentrations by qRT-PCR (See additional file 17 Figure S3) CYP6and4 and CYP6AE11 are highly up-regulated at the highest gossypol concentration.
Let us first inspect the analytical expression of the homography between two consecutive instants.
