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The achieved Gibbs free energy values decrease, in all inspected temperature, if the solvent dielectric constant increases.
The freezing trajectory (Fig. 2C) of the hydrocarbon peak in the Eurosta spectra, on the other hand, remains constant within the inspected temperature range, reflecting the absence of a phase transition in the hydrocarbons (which include non-glycerol and non-sorbitol contributions, e.g. from membrane lipids).
Since the peak nightly temperature was highly variable (apparently associated with the distance between the temperature logger and the nest-bowl), but clearly distinguishable from the background ambient temperature, we visually inspected temperature profiles blind to nest treatment.
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Seven surgical ICU intensivists (four anesthesiologists and three emergency medicine physicians), blinded to group assignment, independently and separately inspected the temperature curves and documented whether abnormal temperature patterns were present.
Because we inspected a relatively small temperature interval, we assumed that Δ H° and Δ S° are fairly temperature-independent.
The reactant concentration, the molar ratio of reactants and the calcination temperature were inspected by homogenous design.
Growth per cycle (GPC) was inspected when changing deposition temperature to 100°C, 200°C, 250°C, and 300°C while the plasma power kept 80 W.
Upon arrival at the laboratory, the lab personnel inspected the sample for temperature and stool volume of at least 3 mL; an accession form was processed.
Sera were prepared from blood collection tubes without anticoagulant after centrifugation at 500 g for 10 minutes at room temperature and inspected visually for any pink hue, which is indicative of hemolysis [ 8], and then, immediately frozen at −80°C.
It is worth mentioning that the number of open substates observed with WT-OccK1 and loop-deletion OccK1 ΔL3 and OccK1 ΔL4 mutants is always preserved in the temperature range inspected in this work (0 25 °C).
The sections were then embedded in a solution containing glycerol and 600 nM 4', 6-diamidino-2-phenylindole (DAPI; Molecular Probes Inc. Eugene, OR, USA) for 5 min at room temperature, then inspected for fluorescence using an Olympus BX60 microscope and photographed using a Mitsubishi digital color printer.
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