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The peptide assignments in the Mascot database search results were then manually inspected for validation.
Data was processed using the InsPecT database search algorithm and the spectra were manually inspected for validation (see flow diagram Figure 3).
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Further, all spectra corresponding to phosphopeptides were manually inspected for correct sequence identification validation and site assignment based on the presence of site determining fragment ions.
After PASS2-HMM validation, they were inspected for which superfamily they belong to and if they could associate to the query member's superfamily.
Component interfaces are then visually inspected for leakage (bubbles).
Electropherograms were visually inspected for heterozygous SNPs.
Data were inspected for normality.
Scanned images were inspected for artefacts.
All scans were inspected for motion artifacts.
All variables were visually inspected for normality.
The alignment was visually inspected for optimality.
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