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DAPI and TUNEL-stained sections were visually inspected for overlapping nuclei within the gastrodermal coral tissue in different areas containing similar number of symbiotic dinoflagellates.
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Conserved regions found by Phastcons were inspected for overlap: if two conserved regions were overlapped by more than 90%% (relative to either one of the two sequences), only the longer region was retained along with its conservation score.
We assessed for statistical heterogeneity between subgroups by visually inspecting the forest plots for overlapping confidence intervals, applying the Chi test (where a P value < 0.10 is considered statistically significant), and by using the I statistic (with values = 40% representing moderate heterogeneity, = 60% substantial heterogeneity, and = 80% considerable heterogeneity).
Individual gene sequences were compared with the homologous sequence of other collembolan species available in GenBank and inspected for the presence of gene overlaps, non-canonical start codons and truncated termination codons, and unusual structures at gene junctions.
Item pairs were plotted (using plot check.iio(FileR))) and inspected visually for overlap and also for 'extreme' items: those lying far from the remaining clusters of items which could also be, artificially, exaggerating IIO.
We determined the presence of statistical heterogeneity across trials by visually inspecting the forest plots to check for overlapping CIs and by means of the Chi² test for heterogeneity with a P value of < 0.10 indicating statistical significance.
Individual chromatograms were inspected for integrity and ambiguous bases were corrected when there was a sequence overlap.
Electropherograms were visually inspected for heterozygous SNPs.
Data were inspected for normality.
Scanned images were inspected for artefacts.
All scans were inspected for motion artifacts.
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CEO of Professional Science Editing for Scientists @ prosciediting.com