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Data was processed using the InsPecT database search algorithm and the spectra were manually inspected for validation (see flow diagram Figure 3).
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At first, based on the inspected database, a three-dimensional finite element analysis was performed by using Abaqus code to analyse the stress distribution and calculate J-integral values of a crack tip through direct definition and stress intensity factor.
We have also inspected the database (named RANDOM database) described recently by Fjell et al. [19] that contents a set of 189 peptides randomly synthesized and experimentally tested, again in uniform conditions.
These were classified into different indications by manually inspecting all database entries (we define indication as a certain symptom which could be treated by the compound rather than the binding of the compound to a certain target).
All the search results were manually inspected and database matches that exceed 99% identity over the length of the aligned region were reported as possible species matches.
Once a given assembled cDNA hit is identified, the user can consult the relational database to inspect the corresponding annotation using the sequence ID.
The Ensembl genome database (GRCh37 assembly, release 64) was used to inspect annotated human p110δ transcripts.
In addition, a database table, named LIPService, was newly established to easily inspect the relationship between intracellular configuration and cell status/morphology.
In order to inspect the intra-gene diversity, an interval mapping tool from the WebQTL database was used.
To address this, a second resource, the NCBI GEO database, was inspected for informative microarray studies.
Every peptide, and not just the ones that deviate from the consensus of a database, is inspected using the ED maps.
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