Fragments were visually inspected, corrected and aligned manually to other species and to each other, using MEGA version 4 [26].
Then he will fax drawings to the factory in Quanzhou and later travel there to inspect and correct the initial prototypes.
However, it is important to mention that in the case of determining the gene structure of pfsr12 orthologues in other Plasmodium species we had to manually inspect and correct the predictions of introns/exons.
Xcalibur was used to inspect the correct monoisotopic peak for glycopeptides on the MS level.
The user-interactive manual annotation editor allows annotators to inspect and correct (where necessary) the automatically generated annotations.
Further, all spectra corresponding to phosphopeptides were manually inspected for correct sequence identification validation and site assignment based on the presence of site determining fragment ions.
Chromatographic peak areas for the formaldehyde isotopologues (light, medium and heavy) of each detected peptide were first extracted using MSQuant [23] and then manually inspected to correct errors from overlapping peaks, missed monoisotopic peaks, etc.
XICs of the peptide with normalized values (≥2) were manually inspected for correct peak assignment.
The ECG tracings were manually inspected to correct for mislabeled beats.
