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The wt Snl1-S fractionated into both soluble and insoluble fraction.
Whatever the method used, the insoluble fraction is systematically excluded.
Insoluble fraction was separated by 1 h centrifugation at 100,000 g.
P, insoluble fraction; S, soluble fraction; F, flow-through; M, protein marker.
The insoluble fraction was removed by centrifugation (16,000 g) for 20 min at 4 °C.
The insoluble fraction (pellet) was solubilized by sonication in lysis buffer containing 2% SDS.
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Immunoelectron microscopy analysis of sarkosyl-insoluble fraction from FAD and control ReN cells.
Main bands from FN at ~220 kDa were identified from smeary bands from DOC-insoluble fraction.
Fontaine, T. et al. Molecular organization of the alkali-insoluble fraction of Aspergillus fumigatus cell wall.
The final pellet was resuspended in 50 µl of dPBS as sarkosyl-insoluble fraction.
Cells (106) and parasites (105) equivalents of the carbonate-insoluble fraction were loaded on SDS-PAGE for immunoblotting.
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