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PLGA constructs contained no appreciable insoluble elastin.
This article describes the most common approaches for purification of insoluble elastin and tropoelastin.
Insoluble elastin preparations tend to calcify, which may be due to calcium-binding microfibrillar (e.g. fibrillin).
This procedure is therefore the method of choice for preparation of insoluble elastin as a biomaterial for tissue engineering.
Our approach to this has been to utilise insoluble elastin in combination with collagen as the basis of a biomimetic scaffold for cardiovascular tissue engineering.
Smooth muscle cells (SMC) were cultured under pulsatile flow conditions in porous tubular scaffolds composed of crosslinked type I insoluble collagen and insoluble elastin.
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The insoluble elastin-dye complex was collected by centrifugation (10,000 g, 10 min), drained of liquid, and destained with 250 µL of a dye dissociation reagent.
Biochemical analyses found that these acellular tissues with distinct porous structures consisted primarily of insoluble collagen, elastin, and tightly bound glycosaminoglycans.
A cell extraction process was employed in the study to remove the cellular components from bovine pericardium, leaving a framework of largely insoluble collagen and elastin.
Native elastin is insoluble and is difficult to extract from tissues, which results in its relatively rare use for the fabrication of vascular tissue engineering scaffolds.
Measuring the release of ELN-441 from elastin-rich tissues emphasized that only MMP-12, and not MMP-9, is able to degrade elastin from insoluble lung and that MMP-12 is faster to degrade elastin from aorta than MMP-9.
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