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SIFT measurements were carried out on an Insight Reader (Evotec-Technologies) with dual-colour excitation at 488 and 633 nm, using a 40 × 1.2 numerical aperture microscope objective (Olympus, Japan) and a pinhole diameter of 70 μm at FIDA setting.
The samples were loaded onto 96-well plates with cover-glass bottom (Evotec-Technologies, Hamburg, Germany) and measured using dual-colour SIFT on an Insight Reader as described [ 2].
Fluorescence correlation spectroscopy (FCS) and scanning for intensely fluorescent targets (SIFTs) measurements for the quantification of α-Synuclein vesicle binding were carried out on an Insight Reader (Evotec-Technologies) with dual colour excitation at 488 and 633 nm as described before [ 31].
SIFT measurements were carried out on an Insight Reader (Evotec-Technologies) with dual-color excitation at 488 and 633 nm, using a 40 × 1.2 numerical aperture microscope objective (Olympus, Japan) and a pinhole diameter of 70 μm at Fluorescence Intensity Distribution Analysis (FIDA) setting.
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SIFT measurements were performed after 24 h on an Insight II Reader (Evotec-Technologies).
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com